Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle
Copyright policy )Rpe65 is necessary for production of 11-cis-vitamin A in the retinal visual cycle
Mutation of RPE65 can cause severe blindness from birth or early childhood, and RPE65 protein is associated with retinal pigment epithelium (RPE) vitamin A metabolism. Here, we show that Rpe65-deficient mice exhibit changes in retinal physiology and biochemistry. Outer segment discs of rod photoreceptors in Rpe65-/- mice are disorganized compared with those of Rpe65+/+ and Rpe65+/- mice. Rod function, as measured by electroretinography, is abolished in Rpe65-/- mice, although cone function remains. Rpe65-/- mice lack rhodopsin, but not opsin apoprotein. Furthermore, all-trans-retinyl esters over-accumulate in the RPE of Rpe65-/- mice, whereas 11-cis-retinyl esters are absent. Disruption of the RPE-based metabolism of all-trans-retinyl esters to 11-cis-retinal thus appears to underlie the Rpe65-/- phenotype, although cone pigment regeneration may be dependent on a separate pathway.
- Medical University of South Carolina United States
- Miami University United States
- Jules Stein Eye Institute United States
- National Institutes of Health United States
- National Institute of Health Pakistan
Mice, Knockout, cis-trans-Isomerases, Rhodopsin, Proteins, Esters, Retina, Mice, Microscopy, Electron, Phenotype, Retinal Rod Photoreceptor Cells, Mutation, Animals, Carrier Proteins, Eye Proteins, Vitamin A, Vision, Ocular
Mice, Knockout, cis-trans-Isomerases, Rhodopsin, Proteins, Esters, Retina, Mice, Microscopy, Electron, Phenotype, Retinal Rod Photoreceptor Cells, Mutation, Animals, Carrier Proteins, Eye Proteins, Vitamin A, Vision, Ocular
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