IFI16 senses DNA forms of the lentiviral replication cycle and controls HIV-1 replication
Copyright policy )IFI16 senses DNA forms of the lentiviral replication cycle and controls HIV-1 replication
Significance HIV-1 is a lentivirus and replicates through a replication cycle involving several DNA forms including ssDNA. Here we report that synthetic DNA oligos corresponding to DNA forms of the lentivirus replication cycle as well as viral DNA are detected by the immunological DNA sensor IFN-inducible protein 16 (IFI16) and stimulate innate immune responses through a pathway dependent on stimulator of IFN genes (STING). Moreover, we show that replication of HIV-1 is elevated in cells with decreased expression of IFI16 or STING. We suggest IFI16 is a sensor for lentivirus DNA in macrophages stimulating innate immune responses, which contribute to early control of the virus.
- National Institute of Health Pakistan
- National Institute of Allergy and Infectious Diseases United States
- National Institutes of Health United States
- Aarhus University Hospital Denmark
- Aarhus University Denmark
Microscopy, Confocal, Macrophages, Membrane Proteins, Nuclear Proteins, Phosphoproteins, Virus Replication, Immunity, Innate, Gene Knockdown Techniques, DNA, Viral, HIV-1, Humans, In Situ Hybridization, Fluorescence, Signal Transduction
Microscopy, Confocal, Macrophages, Membrane Proteins, Nuclear Proteins, Phosphoproteins, Virus Replication, Immunity, Innate, Gene Knockdown Techniques, DNA, Viral, HIV-1, Humans, In Situ Hybridization, Fluorescence, Signal Transduction
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).291 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 1% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!