Role of the plasminogen system in basal adhesion formation and carbon dioxide pneumoperitoneum–enhanced adhesion formation after laparoscopic surgery in transgenic mice
Copyright policy )Role of the plasminogen system in basal adhesion formation and carbon dioxide pneumoperitoneum–enhanced adhesion formation after laparoscopic surgery in transgenic mice
To evaluate the role of plasminogen activator inhibitor-1 (PAI-1), urokinase plasminogen activator (uPA), and tissue-type plasminogen activator (tPA) in adhesion formation after laparoscopic surgery.Prospective, randomized study.Academic research center.Seventy female wild-type and transgenic knockout mice for PAI-1 (PAI-1(-/-)), uPA (uPA(-/-)) or tPA (tPA(-/-)).Standardized lesions to induce peritoneal adhesions were performed during laparoscopy. To evaluate basal adhesions and pneumoperitoneum-enhanced adhesions, the pneumoperitoneum was maintained for 10 minutes or 60 minutes, respectively. Peritoneal biopsy samples were obtained during and after 60 minutes of carbon dioxide pneumoperitoneum.Adhesions were blindly scored after 7 days. Concentrations of PAI-1 and tPA were measured by using enzyme-linked immunosorbent assay.In PAI-1, uPA, and tPA wild-type mice, pneumoperitoneum enhanced adhesions. Compared with wild-type mice, basal adhesions were fewer in PAI-1(-/-) mice and more in uPA(-/-) and tPA(-/-) mice. Pneumoperitoneum did not enhance adhesions in these transgenic mice. PAI-1 concentration increased after 60 minutes of pneumoperitoneum whereas tPA concentration did not change.Impaired fibrinolysis increases basal adhesions. The absence of pneumoperitoneum-enhanced adhesions in PAI-1(-/-), uPA(-/-), and tPA(-/-) mice and the increase in PAI-1 expression indicate that PAI-1 up-regulation by carbon dioxide pneumoperitoneum is a mechanism of pneumoperitoneum-enhanced adhesion formation.
- KU Leuven Belgium
- Katholieke Universiteit Leuven Belgium
Mice, Knockout, Wound Healing, Mice, Transgenic, Tissue Adhesions, Carbon Dioxide, Urokinase-Type Plasminogen Activator, Statistics, Nonparametric, Mice, Inbred C57BL, Mice, Random Allocation, Gene Expression Regulation, Tissue Plasminogen Activator, Plasminogen Activator Inhibitor 1, Animals, Female, Laparoscopy, Prospective Studies, Peritoneum, Pneumoperitoneum, Artificial
Mice, Knockout, Wound Healing, Mice, Transgenic, Tissue Adhesions, Carbon Dioxide, Urokinase-Type Plasminogen Activator, Statistics, Nonparametric, Mice, Inbred C57BL, Mice, Random Allocation, Gene Expression Regulation, Tissue Plasminogen Activator, Plasminogen Activator Inhibitor 1, Animals, Female, Laparoscopy, Prospective Studies, Peritoneum, Pneumoperitoneum, Artificial
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