Interactive Domains for Chaperone Activity in the Small Heat Shock Protein, Human αB Crystallin
Copyright policy )Interactive Domains for Chaperone Activity in the Small Heat Shock Protein, Human αB Crystallin
Protein pin arrays identified seven interactive sequences for chaperone activity in human alphaB crystallin using natural lens proteins, beta(H) crystallin and gammaD crystallin, and in vitro chaperone target proteins, alcohol dehydrogenase and citrate synthase. The N-terminal domain contained two interactive sequences, (9)WIRRPFFPFHSP(20) and (43)SLSPFYLRPPSFLRAP(58). The alpha crystallin core domain contained four interactive sequences, (75)FSVNLDVK(82) (beta3), (113)FISREFHR(120), (131)LTITSSLS(138) (beta8), and (141)GVLTVNGP(148) (beta9). The C-terminal domain contained one interactive sequence, (157)RTIPITRE(164), that included the highly conserved I-X-I/V motif. Two interactive sequences, (73)DRFSVNLDVKHFS(85) and (131)LTITSSLSDGV(141), belonging to the alpha crystallin core domain were synthesized as peptides and assayed for chaperone activity in vitro. Both synthesized peptides inhibited the thermal aggregation of beta(H) crystallin, alcohol dehydrogenase, and citrate synthase in vitro. Five of the seven chaperone sequences identified by the pin arrays overlapped with sequences identified previously as sequences for subunit-subunit interactions in human alphaB crystallin. The results suggested that interactive sequences in human alphaB crystallin have dual roles in subunit-subunit assembly and chaperone activity.
- University of Washington United States
- University of Mary United States
Models, Molecular, Binding Sites, Molecular Sequence Data, Alcohol Dehydrogenase, Protein Array Analysis, alpha-Crystallin B Chain, Nerve Tissue Proteins, Citrate (si)-Synthase, Crystallins, beta-Crystallins, Protein Structure, Tertiary, Intermediate Filament Proteins, Humans, Amino Acid Sequence, gamma-Crystallins, Protein Kinases
Models, Molecular, Binding Sites, Molecular Sequence Data, Alcohol Dehydrogenase, Protein Array Analysis, alpha-Crystallin B Chain, Nerve Tissue Proteins, Citrate (si)-Synthase, Crystallins, beta-Crystallins, Protein Structure, Tertiary, Intermediate Filament Proteins, Humans, Amino Acid Sequence, gamma-Crystallins, Protein Kinases
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