Crystal Structure of the C-terminal Domain of S.cerevisiae eIF5
Copyright policy )Crystal Structure of the C-terminal Domain of S.cerevisiae eIF5
eIF5, a GTPase-activating protein (GAP) specific for eIF2, plays a critical role in pre-initiation complex assembly and correct AUG selection during eukaryotic translation initiation. eIF5 is involved in the formation of the multifactor complex (MFC), an important intermediate of the 43S pre-initiation complex. The C-terminal domain (CTD) of eIF5 functions as the structural core in the MFC assembly. Here we report the 1.5A crystal structure of eIF5-CTD, confirming that eIF5-CTD contains an atypical HEAT motif. In addition, analyzing the electrostatic potential and the distribution of conserved residues on the protein surface, we confirm and suggest some potential regions of interactions between eIF5-CTD and other eIFs. The structure of eIF5-CTD provides useful information in understanding the mechanism of the MFC assembly.
- Chinese Academy of Sciences China (People's Republic of)
- Institute of Biophysics China (People's Republic of)
- University of Science and Technology of China China (People's Republic of)
Models, Molecular, Saccharomyces cerevisiae Proteins, Molecular Sequence Data, Crystallography, X-Ray, Protein Structure, Tertiary, Eukaryotic Initiation Factor-2B, Animals, Humans, Protein Isoforms, Amino Acid Sequence, Eukaryotic Initiation Factor-5, Eukaryotic Initiation Factor-4G, Sequence Alignment
Models, Molecular, Saccharomyces cerevisiae Proteins, Molecular Sequence Data, Crystallography, X-Ray, Protein Structure, Tertiary, Eukaryotic Initiation Factor-2B, Animals, Humans, Protein Isoforms, Amino Acid Sequence, Eukaryotic Initiation Factor-5, Eukaryotic Initiation Factor-4G, Sequence Alignment
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).35 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!