Conditional Depletion of Nuclear Proteins by the Anchor Away System
Copyright policy )Conditional Depletion of Nuclear Proteins by the Anchor Away System
AbstractNuclear proteins play key roles in the regulation of many important cellular processes. In Saccharomyces cerevisiae, many genes encoding nuclear proteins are essential. This unit describes a method termed Anchor Away that can be used to conditionally and rapidly deplete nuclear proteins of interest. It involves conditional export of the protein of interest out of the nucleus and its subsequent sequestration in the cytoplasm. This method can be used to simultaneously deplete multiple proteins from the nucleus. Curr. Protoc. Mol. Biol. 93:13.10B.1‐13.10B.8. © 2011 by John Wiley & Sons, Inc.
- Harvard University United States
Cell Nucleus, Sirolimus, Transcriptional Activation, Cytoplasm, Saccharomyces cerevisiae Proteins, Nuclear Proteins, Saccharomyces cerevisiae, Polymerase Chain Reaction, Anti-Bacterial Agents, Protein Transport, Genetic Techniques, Humans, Genetic Engineering
Cell Nucleus, Sirolimus, Transcriptional Activation, Cytoplasm, Saccharomyces cerevisiae Proteins, Nuclear Proteins, Saccharomyces cerevisiae, Polymerase Chain Reaction, Anti-Bacterial Agents, Protein Transport, Genetic Techniques, Humans, Genetic Engineering
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