Syk is a dual-specificity kinase that self-regulates the signal output from the B-cell antigen receptor
Copyright policy )Syk is a dual-specificity kinase that self-regulates the signal output from the B-cell antigen receptor
Upon B-cell activation, the signaling subunits Ig-α and Ig-β of the B-cell antigen receptor become phosphorylated not only on tyrosines but also on serine residues. Using a specific antibody, we show that serine 197 (S197) in the cytoplasmic tail of Ig-α is phosphorylated upon B-cell antigen receptor activation, and that this modification inhibits the signal output of the B-cell antigen receptor. Surprisingly, we found that the well-known protein tyrosine kinase Syk (spleen tyrosine kinase) phosphorylates S197 on Ig-α, thus not only activating but also inhibiting signaling from the B-cell antigen receptor. This finding identifies Syk as a dual-specificity kinase and establishes a previously unexplored paradigm for the self-regulation of biological signaling processes.
- Max Planck Society Germany
- University of Freiburg Germany
Mice, Knockout, Models, Molecular, B-Lymphocytes, Mice, Inbred BALB C, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Receptors, Antigen, B-Cell, Mice, Transgenic, Protein-Tyrosine Kinases, Recombinant Proteins, Cell Line, Mice, Mutation, Serine, Animals, Drosophila, Amino Acid Sequence, Phosphorylation, CD79 Antigens, Signal Transduction
Mice, Knockout, Models, Molecular, B-Lymphocytes, Mice, Inbred BALB C, Molecular Sequence Data, Intracellular Signaling Peptides and Proteins, Receptors, Antigen, B-Cell, Mice, Transgenic, Protein-Tyrosine Kinases, Recombinant Proteins, Cell Line, Mice, Mutation, Serine, Animals, Drosophila, Amino Acid Sequence, Phosphorylation, CD79 Antigens, Signal Transduction
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).48 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!