Self-medication raises its occurrence in many situations in our everyday lives, which is an inappropriate and harmful practice. A research framework was conducted to evaluate the awareness, attitude and practice of self-medication among the CHARUSAT University staff members. Among the staff members, a pre-validated questionnaire was circulated. Using Microsoft Excel, information was gathered and evaluated and the results were represented as numbers and percentages. A total of 136 staff-members voluntarily took part in the study. We found that source of information of the drugs used for self-medication were “previous prescription (77.7%)” and the source of drugs was “medical store (78.8%)”. Only (34.3%) staff-members accepted the fact that they always visited a qualified practitioner whenever they got sick. Most of the staff-members took self-medication for headache (73.5%) followed by cough, cold, sore throat (73.5%) and fever (56.6%). Out of total 136 staff-members, most of them took Analgesics (61.8%) as self-medication followed by lozenges (66.2%). For almost 66.9 % of university staff members, self-medication was common. They provided a reason that “no need to visit a doctor for minor illnesses”. 73.5 % of the population has taken cough and cold preparations as self-medication.