Absence of Apparent Phenotype in Mice Lacking Cdc25C Protein Phosphatase
Copyright policy )Absence of Apparent Phenotype in Mice Lacking Cdc25C Protein Phosphatase
The Cdc25 family of protein phosphatases positively regulate the cell division cycle by activating cyclin-dependent protein kinases. In humans and rodents, three Cdc25 family members denoted Cdc25A, -B, and -C have been identified. The murine forms of Cdc25 exhibit distinct patterns of expression both during development and in adult mouse tissues. In order to determine unique contributions made by the Cdc25C protein phosphatase to embryonic and adult cell cycles, mice lacking Cdc25C were generated. We report that Cdc25C(-/-) mice are viable and do not display any obvious abnormalities. Among adult tissues in which Cdc25C is detected, its transcripts are most abundant in testis, followed by thymus, ovary, spleen, and intestine. Mice lacking Cdc25C were fertile, indicating that Cdc25C does not contribute an essential function during spermatogenesis or oogenesis in the mouse. T- and B-cell development was also found to be normal in Cdc25C(-/-) mice, and Cdc25C(-/-) mouse splenic T and B cells exhibited normal proliferative responses in vitro. Finally, the phosphorylation status of Cdc2, the timing of entry into mitosis, and the cellular response to DNA damage were unperturbed in mouse embryo fibroblasts lacking Cdc25C. These findings indicate that Cdc25A and/or Cdc25B may compensate for loss of Cdc25C in the mouse.
- Washington University in St. Louis School of Medicine United States
- Washington University in St. Louis United States
- Howard Hughes Medical Institute United States
- University of Mary United States
- Howard Hughes Medical Institute
Male, Mice, Knockout, B-Lymphocytes, Base Sequence, T-Lymphocytes, Cell Cycle, Gene Expression Regulation, Developmental, Cell Cycle Proteins, Mice, Inbred C57BL, Mice, Fertility, Oogenesis, Phenotype, Gene Targeting, Animals, Humans, Female, Tissue Distribution, Spermatogenesis, DNA Primers
Male, Mice, Knockout, B-Lymphocytes, Base Sequence, T-Lymphocytes, Cell Cycle, Gene Expression Regulation, Developmental, Cell Cycle Proteins, Mice, Inbred C57BL, Mice, Fertility, Oogenesis, Phenotype, Gene Targeting, Animals, Humans, Female, Tissue Distribution, Spermatogenesis, DNA Primers
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).173 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!