Amphiregulin enhances intercellular adhesion molecule-1 expression and promotes tumor metastasis in human osteosarcoma
Copyright policy )Amphiregulin enhances intercellular adhesion molecule-1 expression and promotes tumor metastasis in human osteosarcoma
Osteosarcoma is a common, high malignant, and metastatic bone cancer. Amphiregulin (AREG) has been associated with cancer cellular activities. However, the effect of AREG on metastasis activity in human osteosarcoma cells has yet to be determined. We determined that AREG increases the expression of intercellular adhesion molecule-1 (ICAM-1) through PI3K/Akt signaling pathway via its interaction with the epidermal growth factor receptor, thus resulting in the enhanced cell migration of osteosarcoma. Furthermore, AREG stimulation increased the association of NF-κB to ICAM-1 promoter which then up-regulated ICAM-1 expression. Finally, we observed that shRNA silencing of AREG decreased osteosarcoma metastasis in vivo. Our findings revealed a relationship between osteosarcoma metastatic potential and AREG expression and the modulating effect of AREG on ICAM-1 expression.
- National Taiwan University Hospital Taiwan
- Memorial Hospital of South Bend United States
- National Taiwan University of Arts Taiwan
Male, Chromatin Immunoprecipitation, EGF Family of Proteins, Osteosarcoma, Lung Neoplasms, Blotting, Western, NF-kappa B, Apoptosis, Bone Neoplasms, Mice, SCID, Intercellular Adhesion Molecule-1, Amphiregulin, ErbB Receptors, Immunoenzyme Techniques, Mice, Phosphatidylinositol 3-Kinases, Cell Movement, Animals, Humans, Cell Proliferation
Male, Chromatin Immunoprecipitation, EGF Family of Proteins, Osteosarcoma, Lung Neoplasms, Blotting, Western, NF-kappa B, Apoptosis, Bone Neoplasms, Mice, SCID, Intercellular Adhesion Molecule-1, Amphiregulin, ErbB Receptors, Immunoenzyme Techniques, Mice, Phosphatidylinositol 3-Kinases, Cell Movement, Animals, Humans, Cell Proliferation
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