α‐Actinin4 nuclear translocation mediates gonadotropin‐releasing hormone stimulation of follicle‐stimulating hormone β‐subunit gene transcription in LβT2 cells
Copyright policy )α‐Actinin4 nuclear translocation mediates gonadotropin‐releasing hormone stimulation of follicle‐stimulating hormone β‐subunit gene transcription in LβT2 cells
Gonadotropin‐releasing hormone (GnRH) regulates the synthesis and secretion of follicle‐stimulating hormone (FSH) by stimulating the transcription of Fshβ gene. Our iTRAQ quantitative proteomics result showed that the abundance of α‐actinin4 (ACTN4) increased in the nuclei of LβT2 cells upon GnRH induction. Using RNA interference, reverse transcription and real‐time PCR, luciferase and transient transfection assays, we proved that ACTN4 is involved in the regulation of mouse Fshβ gene (mFshβ) transcription and its C‐terminal calmodulin (CaM)‐like domain is crucial for this process. Our study suggests that ACTN4 nuclear translocation mediates GnRH stimulation of mFshβ gene transcription.
- National University of Singapore Singapore
- Temasek Life Sciences Laboratory Singapore
- National University of Singapore Libraries Singapore
Calmodulin-like domain, Cell Nucleus, Proteomics, 570, Base Sequence, Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Nuclear translocation, Real-Time Polymerase Chain Reaction, ACTN4, Cell Line, Gonadotropin-Releasing Hormone, Mice, Protein Transport, Fshβ, GnRH, 616, Follicle Stimulating Hormone, beta Subunit, Animals, Actinin, Promoter Regions, Genetic, DNA Primers
Calmodulin-like domain, Cell Nucleus, Proteomics, 570, Base Sequence, Transcription, Genetic, Reverse Transcriptase Polymerase Chain Reaction, Nuclear translocation, Real-Time Polymerase Chain Reaction, ACTN4, Cell Line, Gonadotropin-Releasing Hormone, Mice, Protein Transport, Fshβ, GnRH, 616, Follicle Stimulating Hormone, beta Subunit, Animals, Actinin, Promoter Regions, Genetic, DNA Primers
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