Developmentally Regulated Mouse Gene NK10 Encodes a Zinc Finger Repressor Protein with Differential DNA-Binding Domains
Copyright policy )Developmentally Regulated Mouse Gene NK10 Encodes a Zinc Finger Repressor Protein with Differential DNA-Binding Domains
Using oligonucleotides complementary to the conserved inter-finger region of a variety of previously described zinc finger-encoding genes, a novel mouse gene was cloned and characterized. The gene is localized on chromosome 8 and comprises five exons. Its corresponding mRNA is developmentally regulated in various tissues and includes an open reading frame encoding a protein of 72,422 daltons. It shares amino-terminal homologies with human KRAB (or FPB) boxes, and contains 13 zinc fingers of the C2-H2 type. The NK10 KRAB domains exhibit repressing activity when tested in GAL4 fusion protein assays. Cloning of putative target sequences revealed that the individual domains differentially contribute to zinc-dependent target DNA binding.
- University of Cologne Germany
Base Sequence, Recombinant Fusion Proteins, Molecular Sequence Data, Chromosome Mapping, Gene Expression Regulation, Developmental, Zinc Fingers, Exons, Sequence Analysis, DNA, DNA-Binding Proteins, Repressor Proteins, Mice, Open Reading Frames, Animals, Newborn, Organ Specificity, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular
Base Sequence, Recombinant Fusion Proteins, Molecular Sequence Data, Chromosome Mapping, Gene Expression Regulation, Developmental, Zinc Fingers, Exons, Sequence Analysis, DNA, DNA-Binding Proteins, Repressor Proteins, Mice, Open Reading Frames, Animals, Newborn, Organ Specificity, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular
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