Somatostatin sst2receptors inhibit peristalsis in the rat and mouse jejunum
Copyright policy )Somatostatin sst2receptors inhibit peristalsis in the rat and mouse jejunum
Somatostatin [somatotropin release-inhibitory factor (SRIF)] has widespread actions throughout the gastrointestinal tract, but the receptor mechanisms involved are not fully characterized. We have examined the effect of selective SRIF-receptor ligands on intestinal peristalsis by studying migrating motor complexes (MMCs) in isolated segments of jejunum from rats, mice, and sst2-receptor knockout mice. MMCs were recorded in 4- to 5-cm segments of jejunum mounted horizontally in vitro. MMCs occurred in rat and mouse jejunum with intervals of 104.4 ± 10 and 131.2 ± 8 s, respectively. SRIF, octreotide, and BIM-23027 increased the interval between MMCs, an effect fully or partially antagonized by the sst2-receptor antagonist Cyanamid154806. A non-sst2receptor-mediated component was evident in mouse as confirmed by the observation of an inhibitory action of SRIF in sst2knockout tissue. Blocking nitric oxide generation abolished the response to SRIF in rat but not mouse jejunum. sst2Receptors mediate inhibition of peristalsis in both rat and mouse jejunum, but a non-sst2component also exists in the mouse. Nitrergic mechanisms are differentially involved in rat and mouse jejunum.
- GlaxoSmithKline (United States) United States
- Nevada System of Higher Education United States
- University of Sheffield United Kingdom
- Biotechnology and Biological Sciences Research Council United Kingdom
- Cellzome, GSK, Middlesex, UK.
Atropine, Male, Mice, Knockout, Nifedipine, Octreotide, Peptides, Cyclic, Rats, Mice, Inbred C57BL, Mice, Jejunum, NG-Nitroarginine Methyl Ester, Pressure, Animals, Female, Peristalsis, Receptors, Somatostatin, Enzyme Inhibitors, Nitric Oxide Synthase, Somatostatin, Muscle Contraction
Atropine, Male, Mice, Knockout, Nifedipine, Octreotide, Peptides, Cyclic, Rats, Mice, Inbred C57BL, Mice, Jejunum, NG-Nitroarginine Methyl Ester, Pressure, Animals, Female, Peristalsis, Receptors, Somatostatin, Enzyme Inhibitors, Nitric Oxide Synthase, Somatostatin, Muscle Contraction
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