G‐quadruplex‐forming nucleic acids interact with splicing factor 3B subunit 2 and suppress innate immune gene expression
Copyright policy )G‐quadruplex‐forming nucleic acids interact with splicing factor 3B subunit 2 and suppress innate immune gene expression
AbstractG‐quadruplex (G4), a non‐canonical higher‐order structure formed by guanine‐rich nucleic acid sequences, affects various genetic events in cis, including replication, transcription and translation. Whereas up‐regulation of innate immune/interferon‐stimulated genes (ISGs) is implicated in cancer progression, G4‐forming oligonucleotides that mimic telomeric repeat‐containing RNA suppress ISG induction in three‐dimensional (3D) culture of cancer cells. However, it is unclear how G4 suppresses ISG expression in trans. In this study, we found that G4 binding to splicing factor 3B subunit 2 (SF3B2) down‐regulated STAT1 phosphorylation and ISG expression in 3D‐cultured cancer cells. Liquid chromatography‐tandem mass spectrometry analysis identified SF3B2 as a G4‐binding protein. Either G4‐forming oligonucleotides or SF3B2 knockdown suppressed ISG induction, whereas Phen‐DC3, a G4‐stabilizing compound, reversed the inhibitory effect of G4‐forming oligonucleotides on ISG induction. Phen‐DC3 inhibited SF3B2 binding to G4 in vitro. SF3B2‐mediated ISG induction appeared to occur independently of RNA splicing because SF3B2 knockdown did not affect pre‐mRNA splicing under the experimental conditions, and pharmacological inhibition of splicing by pladienolide B did not repress ISG induction. These observations suggest that G4 disrupts the ability of SF3B2 to induce ISGs in cancer. We propose a new mode for gene regulation, which employs G4 as an inhibitory trans‐element.
Fused-Ring Compounds, RNA Splicing, Oligonucleotides, Original Articles, Ligands, Models, Biological, Immunity, Innate, G-Quadruplexes, Gene Ontology, STAT1 Transcription Factor, Gene Expression Regulation, Cell Line, Tumor, Gene Knockdown Techniques, Nucleic Acids, Cytokines, Humans, RNA Splicing Factors, Ubiquitins, Protein Binding, Signal Transduction
Fused-Ring Compounds, RNA Splicing, Oligonucleotides, Original Articles, Ligands, Models, Biological, Immunity, Innate, G-Quadruplexes, Gene Ontology, STAT1 Transcription Factor, Gene Expression Regulation, Cell Line, Tumor, Gene Knockdown Techniques, Nucleic Acids, Cytokines, Humans, RNA Splicing Factors, Ubiquitins, Protein Binding, Signal Transduction
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