Microarray Analysis of Genes that Respond to γ-Irradiation in Arabidopsis
Copyright policy )Microarray Analysis of Genes that Respond to γ-Irradiation in Arabidopsis
To reveal the signal transduction mechanism of the response to stress in the form of active oxygen species, we used a microarray system to analyze gene expression patterns 2 or 24 h after gamma-irradiation of Arabidopsis. gamma-Irradiation induces several signal transduction and metabolite genes. By analysis of cis-elements located on the promoter region of the gamma-responsive genes, we have also found several cis-elements related to various signal transduction systems. We also analyzed the pleiotropic mutant ttg1-1, which has a dramatically altered physiological response to gamma-irradiation. By comparing the gene expression patterns of wild-type (Ler) and ttg1-1 mutant plants after gamma-irradiation, we identified various TTG1-regulated gamma-response genes. Analysis of the cis-elements in the promoter region of the gamma-responsive genes also revealed that the many transcription factors interacting with TTG1 protein (WD40 protein) are related to the gamma-responsive gene expression.
- Mayo Clinic United States
- National Agriculture and Food Research Organization Japan
- Institute of Agrobiological Sciences Japan
- Food Research Institute Japan
Arabidopsis Proteins, Gamma Rays, RNA, Plant, Mutation, Arabidopsis, Gene Expression, Genes, Plant, Oligonucleotide Array Sequence Analysis
Arabidopsis Proteins, Gamma Rays, RNA, Plant, Mutation, Arabidopsis, Gene Expression, Genes, Plant, Oligonucleotide Array Sequence Analysis
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