AbstractBackgroundThe ability of the gut to detect nutrients is critical to the regulation of gut hormone secretion, food intake, and postprandial blood glucose control. Ingested nutrients are detected by specific gut chemosensors. However, knowledge of these chemosensors has primarily been derived from the intestine, while available information on gastric chemosensors is limited. This study aimed to investigate the nutrient‐sensing repertoire of the mouse stomach with particular emphasis on ghrelin cells.MethodsQuantitative RT‐PCR was used to determine mRNA levels of nutrient chemosensors (protein: G protein–coupled receptor 93 [GPR93], calcium‐sensing receptor [CaSR], metabotropic glutamate receptor type 4 [mGluR4]; fatty acids: CD36, FFAR2&4; sweet/umami taste: T1R3), taste transduction components (TRPM5, GNAT2&3), and ghrelin and ghrelin‐processing enzymes (PC1/3, ghrelin O‐acyltransferase [GOAT]) in the gastric corpus and antrum of adult male C57BL/6 mice. Immunohistochemistry was performed to assess protein expression of chemosensors (GPR93, T1R3, CD36, and FFAR4) and their co‐localization with ghrelin.Key ResultsMost nutrient chemosensors had higher mRNA levels in the antrum compared to the corpus, except for CD36, GNAT2, ghrelin, and GOAT. Similar regional distribution was observed at the protein level. At least 60% of ghrelin‐positive cells expressed T1R3 and FFAR4, and over 80% expressed GPR93 and CD36.Conclusions and InferencesThe cellular mechanisms for the detection of nutrients are expressed in a region‐specific manner in the mouse stomach and gastric ghrelin cells. These gastric nutrient chemosensors may play a role modulating gastrointestinal responses, such as the inhibition of ghrelin secretion following food intake.