Galectin-3 Secreted by Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Reduces Amyloid-Beta42 Neurotoxicity In Vitro
Copyright policy )Galectin-3 Secreted by Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Reduces Amyloid-Beta42 Neurotoxicity In Vitro
In this study, we found that expression and secretion of galectin‐3 (GAL‐3) were upregulated by amyloid‐β42 (Aβ42) exposure in human umbilical cord blood‐derived mesenchymal stem cell (hUCB‐MSC) without cell death. Aβ42‐exposed rat primary cortical neuronal cells co‐treated with recombinant GAL‐3 were protected from neuronal death in a dose‐dependent manner. hUCB‐MSCs were cocultured with Aβ42‐exposed rat primary neuronal cells or the neuroblastoma cell line, SH‐SY5Y in a Transwell chamber. Coculture of hUCB‐MSCs reduced cell death of Aβ42‐exposed neurons and SH‐SY5Y cells. This neuroprotective effect of hUCB‐MSCs was reduced significantly by GAL‐3 siRNA. These data suggested that hUCB‐MSC‐derived GAL‐3 is a survival factor against Aβ42 neurotoxicity.
- BIOMEDICAL RESEARCH INSTITUTE
- Biomedical Research Institute United States
- Catholic University of Korea Korea (Republic of)
- Mogam Institute for Biomedical Research Korea (Republic of)
- Département de Physiologie Switzerland
Neurons, Amyloid beta-Peptides, Galectin 3, Neurotoxins, Biophysics, Mesenchymal Stem Cells, hUCB-MSC, Fetal Blood, Neuroprotection, Coculture Techniques, Peptide Fragments, Cell Line, Rats, Up-Regulation, Neuroprotective Agents, Gene Knockdown Techniques, Galectin-3, Animals, Humans, Amyloid-β, RNA, Small Interfering, Cells, Cultured
Neurons, Amyloid beta-Peptides, Galectin 3, Neurotoxins, Biophysics, Mesenchymal Stem Cells, hUCB-MSC, Fetal Blood, Neuroprotection, Coculture Techniques, Peptide Fragments, Cell Line, Rats, Up-Regulation, Neuroprotective Agents, Gene Knockdown Techniques, Galectin-3, Animals, Humans, Amyloid-β, RNA, Small Interfering, Cells, Cultured
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