Identification of essential components of the S. cerevisiae kinetochore
Copyright policy )Identification of essential components of the S. cerevisiae kinetochore
We have designed and utilized two in vivo assays of kinetochore integrity in S. cerevisiae. One assay detects relaxation of a transcription block formed at centromeres; the other detects an increase in the mitotic stability of a dicentric test chromosome. ctf13-30 and ctf14-42 were identified as putative kinetochore mutants by both assays. CTF14 is identical to NDC10/CBF2, a recently identified essential gene that encodes a 110 kd kinetochore component. CTF13 is an essential gene that encodes a predicted 478 amino acid protein with no homology to known proteins. ctf13 mutants missegregate chromosomes at permissive temperature and transiently arrest at nonpermissive temperature as large-budded cells with a G2 DNA content and a short spindle. Antibodies recognizing epitope-tagged CTF13 protein decrease the electrophoretic mobility of a CEN DNA-protein complex formed in vitro. Together, the genetic and biochemical data indicate that CTF13 is an essential kinetochore protein.
- Johns Hopkins Medicine United States
- University of California, San Francisco United States
- Johns Hopkins University United States
Saccharomyces cerevisiae Proteins, Base Sequence, Centromere, Molecular Sequence Data, Nuclear Proteins, Saccharomyces cerevisiae, Article, DNA-Binding Proteins, Fungal Proteins, Phenotype, Mutation, Amino Acid Sequence, Cloning, Molecular, Kinetochores
Saccharomyces cerevisiae Proteins, Base Sequence, Centromere, Molecular Sequence Data, Nuclear Proteins, Saccharomyces cerevisiae, Article, DNA-Binding Proteins, Fungal Proteins, Phenotype, Mutation, Amino Acid Sequence, Cloning, Molecular, Kinetochores
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