Coincident Activation of Th2 T Cells with Onset of the Disease and Differential Expression of GRO-Gamma in Peripheral Blood Leukocytes in Minimal Change Disease
Copyright policy )Coincident Activation of Th2 T Cells with Onset of the Disease and Differential Expression of GRO-Gamma in Peripheral Blood Leukocytes in Minimal Change Disease
<i>Background:</i> Involvement of Th2 T cells/NFĸB in minimal change disease (MCD) has been postulated. A promising but unconfirmed glomerular permeability factor (GPF) from MCD T cells has been described. We explored whether GPF was the consequence of Th2 cell activation. <i>Methods:</i> Peripheral blood leukocytes (PBL) from 16 MCD patients and 7 normal controls were analyzed and the results were statistically compared. <i>Results:</i> Flow cytometry demonstrated a significant expansion of CD4+ T cell population and dramatically increased CD69+ cells among CD4+ T cells in MCD, suggesting coincident activation of T cells with onset of the disease. RT-PCR on RNA from either freshly isolated PBL or post in vitroactivation showed high-level expression of the Th2 cytokine interleukin-4 in all MCD patients. Importantly, both antibody microarray assay on sera and RT-PCR on mRNA of PBL revealed expression of a CXC chemokine GRO-γ (growth-related oncogene) in all MCD patients as compared with one of 7 controls. <i>Conclusions:</i> Our results reveal an association between onset of MCD and activation of Th2 cells. The GRO family has been implicated in the function of endothelial cells, and its expression is under NFĸB regulation. Thus, GRO-γ is a promising candidate for Th2-associated GPF in MCD.
- Baylor College of Medicine United States
- The University of Texas Health Science Center at Houston United States
- Methodist Hospital United States
- University of Florida United States
- Dignity Health United States
Antigens, Differentiation, T-Lymphocyte, Male, Nephrosis, Lipoid, Gene Expression, Lymphocyte Activation, Permeability, CD4 Lymphocyte Count, Th2 Cells, Antigens, CD, Case-Control Studies, Child, Preschool, CD4 Antigens, Glomerular Basement Membrane, Cytokines, Humans, Female, Lectins, C-Type, Child, Chemokines, CXC
Antigens, Differentiation, T-Lymphocyte, Male, Nephrosis, Lipoid, Gene Expression, Lymphocyte Activation, Permeability, CD4 Lymphocyte Count, Th2 Cells, Antigens, CD, Case-Control Studies, Child, Preschool, CD4 Antigens, Glomerular Basement Membrane, Cytokines, Humans, Female, Lectins, C-Type, Child, Chemokines, CXC
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).14 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!