Upregulation by KCl Treatment of Eukaryotic Translation Elongation Factor 1A (eEF1A) mRNA in the Dendrites of Cultured Rat Hippocampal Neurons
Copyright policy )Upregulation by KCl Treatment of Eukaryotic Translation Elongation Factor 1A (eEF1A) mRNA in the Dendrites of Cultured Rat Hippocampal Neurons
Activity-dependent local translation in the dendrites of brain neurons plays an important role in the synapse-specific provision of proteins necessary for strengthening synaptic connections. In this study we carried out combined fluorescence in situ hybridization (FISH) and immunocytochemistry (IC) and showed that more than half of the eukaryotic elongation factor 1A (eEF1A) mRNA clusters overlapped with or were immediately adjacent to clusters of PSD-95, a postsynaptic marker, in the dendrites of cultured rat hippocampal neurons. Treatment of the neurons with KCl increased the density of the dendritic eEF1A mRNA clusters more than two-fold. FISH combined with IC revealed that the KCl treatment increased the density of eEF1A mRNA clusters that overlapped with or were immediately adjacent to PSD-95 clusters. These results indicate that KCl treatment increases both the density of eEF1A mRNA clusters and their synaptic association in dendrites of cultured neurons.
- University of Connecticut United States
- Inje University Korea (Republic of)
- Kyungpook National University Korea (Republic of)
- Dongguk University Korea (Republic of)
Neurons, Microscopy, Confocal, Intracellular Signaling Peptides and Proteins, Synaptic Membranes, Biological Transport, Active, Membrane Proteins, Dendrites, Hippocampus, Potassium Chloride, Rats, Up-Regulation, DNA-Binding Proteins, Rats, Sprague-Dawley, Animals, RNA, Messenger, Disks Large Homolog 4 Protein, Cells, Cultured, In Situ Hybridization, Fluorescence
Neurons, Microscopy, Confocal, Intracellular Signaling Peptides and Proteins, Synaptic Membranes, Biological Transport, Active, Membrane Proteins, Dendrites, Hippocampus, Potassium Chloride, Rats, Up-Regulation, DNA-Binding Proteins, Rats, Sprague-Dawley, Animals, RNA, Messenger, Disks Large Homolog 4 Protein, Cells, Cultured, In Situ Hybridization, Fluorescence
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).12 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!