C3PO, an Endoribonuclease That Promotes RNAi by Facilitating RISC Activation
Copyright policy )C3PO, an Endoribonuclease That Promotes RNAi by Facilitating RISC Activation
RNA Wars During RNA interference (RNAi), the Dicer endonuclease generates small interfering (si)RNAs that, with the help of the protein R2D2, are loaded into the siRNA-induced silencing complex (RISC). Using siRNAs as guides, RISC, and specifically its Argonaute subunit, targets complementary RNAs for destruction. In order to identify other components of the RISC complex, Liu et al. (p. 750 ) reconstituted the core RISC activity, using purified Drosophila Dicer, R2D2, and Ago-2. The protein C3PO (component 3 promoter of RISC), which consists of heterodimer of Translin and Translin-associated factor X (Trax), was found to enhance RISC activity in this system, and in vivo, with the Trax endonuclease activity activating RISC through the removal of siRNA passenger strand cleavage products.
- The University of Texas Southwestern Medical Center United States
- Emory University United States
- Howard Hughes Medical Institute United States
Models, Molecular, Ribonuclease III, Protein Conformation, Molecular Sequence Data, RNA-Binding Proteins, Recombinant Proteins, DNA-Binding Proteins, Drosophila melanogaster, Catalytic Domain, Argonaute Proteins, Mutation, Animals, Drosophila Proteins, RNA-Induced Silencing Complex, RNA Interference, Amino Acid Sequence, RNA, Small Interfering, Carrier Proteins, RNA Helicases, RNA, Double-Stranded
Models, Molecular, Ribonuclease III, Protein Conformation, Molecular Sequence Data, RNA-Binding Proteins, Recombinant Proteins, DNA-Binding Proteins, Drosophila melanogaster, Catalytic Domain, Argonaute Proteins, Mutation, Animals, Drosophila Proteins, RNA-Induced Silencing Complex, RNA Interference, Amino Acid Sequence, RNA, Small Interfering, Carrier Proteins, RNA Helicases, RNA, Double-Stranded
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