Expression of Leptin Receptor Isoforms and Effects of Leptin on the Proliferation and Hormonal Secretion in Human Pituitary Adenomas
Copyright policy )Expression of Leptin Receptor Isoforms and Effects of Leptin on the Proliferation and Hormonal Secretion in Human Pituitary Adenomas
<i>Objective:</i> To pursue whether leptin regulates anterior pituitary cells, we studied the ex vivo expression of several isoforms of the leptin receptor (OB-R) as well as the in vitro effects of leptin administration in human pituitary adenomas. <i>Methods:</i> OB-R mRNA expression and in vitro response to leptin were studied in 39 pituitary macroadenomas. <i>Results:</i> All 4 OB-R subtypes were expressed in most adenomas. The expression was significantly more pronounced in GH-secreting adenomas as compared to non-functioning tumor cells (p < 0.05). Leptin administration in vitro did not significantly influence cell proliferation or the secretion of GH, FSH, LH or α-subunit. <i>Conclusions:</i> (1) Several isoforms of the OB-R, including the signal transducing full-length receptor, are expressed in most human pituitary adenomas. (2) This expression ex vivo is not associated with significant effects of leptin in vitro.
- Aarhus University Hospital Denmark
- Odense University Hospital Denmark
- Molecular Pathology Laboratory Network (United States) United States
Adenoma, Leptin, Male, Human Growth Hormone, Reverse Transcriptase Polymerase Chain Reaction, Receptors, Cell Surface, In Vitro Techniques, Luteinizing Hormone, Middle Aged, Gene Expression Regulation, Neoplastic, Isomerism, Glycoprotein Hormones, alpha Subunit, Tumor Cells, Cultured, Humans, Receptors, Leptin, Female, Pituitary Neoplasms, RNA, Messenger, Follicle Stimulating Hormone, Cell Division
Adenoma, Leptin, Male, Human Growth Hormone, Reverse Transcriptase Polymerase Chain Reaction, Receptors, Cell Surface, In Vitro Techniques, Luteinizing Hormone, Middle Aged, Gene Expression Regulation, Neoplastic, Isomerism, Glycoprotein Hormones, alpha Subunit, Tumor Cells, Cultured, Humans, Receptors, Leptin, Female, Pituitary Neoplasms, RNA, Messenger, Follicle Stimulating Hormone, Cell Division
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