Genome-wide analysis of nuclear mRNA export pathways in Drosophila
Copyright policy )Genome-wide analysis of nuclear mRNA export pathways in Drosophila
NXF1, p15 and UAP56 are essential nuclear mRNA export factors. The fraction of mRNAs exported by these proteins or via alternative pathways is unknown. We have analyzed the relative abundance of nearly half of the Drosophila transcriptome in the cytoplasm of cells treated with the CRM1 inhibitor leptomycin-B (LMB) or depleted of export factors by RNA interference. While the vast majority of mRNAs were unaffected by LMB, the levels of most mRNAs were significantly reduced in cells depleted of NXF1, p15 or UAP56. The striking similarities of the mRNA expression profiles in NXF1, p15 and UAP56 knockdowns show that these proteins act in the same pathway. The broad effect on mRNA levels observed in these cells indicates that the functioning of this pathway is required for export of most mRNAs. Nonetheless, a set of mRNAs whose export was unaffected by the depletions and some requiring NXF1:p15 but not UAP56 were identified. In addition, our analysis revealed a feedback loop by which a block to mRNA export triggers the upregulation of genes involved in this process.
- European Molecular Biology Laboratory Germany
- European Bioinformatics Institute United Kingdom
Cell Nucleus, Nucleocytoplasmic Transport Proteins, Genome, Gene Expression Profiling, Active Transport, Cell Nucleus, RNA-Binding Proteins, Receptors, Cytoplasmic and Nuclear, Exportin 1 Protein, Karyopherins, Drosophila melanogaster, Gene Expression Regulation, Animals, Drosophila Proteins, Humans, RNA, Messenger, Oligonucleotide Array Sequence Analysis
Cell Nucleus, Nucleocytoplasmic Transport Proteins, Genome, Gene Expression Profiling, Active Transport, Cell Nucleus, RNA-Binding Proteins, Receptors, Cytoplasmic and Nuclear, Exportin 1 Protein, Karyopherins, Drosophila melanogaster, Gene Expression Regulation, Animals, Drosophila Proteins, Humans, RNA, Messenger, Oligonucleotide Array Sequence Analysis
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).138 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!