cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo
Copyright policy )cDNA cloning and mRNA expression of Transformer 2 (Tra 2) in chicken embryo
We report the cloning of a chicken Transformer 2 (Tra 2) cDNA that encodes a protein of 289 amino acids which are 97.9% identical to those of mammalian splicing factor, Tra 2. Tra 2 mRNA was expressed in chicken embryonic tissues and was observed as a band of 1.5 kb by Northern blot analysis. Whole mount in situ hybridization showed an mRNA expression of Tra 2 in telencephalon, mandible, hyoid arch, wing and leg buds as early as day 3.5 of incubation. These results suggest that the Tra 2 gene may play a role in organogenesis in the chicken embryo.
Telencephalon, DNA, Complementary, Base Sequence, Limb Buds, Molecular Sequence Data, RNA-Binding Proteins, Chick Embryo, Mandible, Blotting, Northern, Ribonucleoproteins, Animals, Drosophila Proteins, Wings, Animal, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Sequence Alignment, In Situ Hybridization
Telencephalon, DNA, Complementary, Base Sequence, Limb Buds, Molecular Sequence Data, RNA-Binding Proteins, Chick Embryo, Mandible, Blotting, Northern, Ribonucleoproteins, Animals, Drosophila Proteins, Wings, Animal, Amino Acid Sequence, RNA, Messenger, Cloning, Molecular, Sequence Alignment, In Situ Hybridization
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