Expression of Hepatocyte Growth Factor/Scatter Factor, Its Receptor,c-met, and Tissue-Type Plasminogen Activator during Development of the Murine Olfactory System
Copyright policy )Expression of Hepatocyte Growth Factor/Scatter Factor, Its Receptor,c-met, and Tissue-Type Plasminogen Activator during Development of the Murine Olfactory System
The expression of hepatocyte growth factor/scatter factor (HGF/SF) and its receptor, thec-metproto-oncogene product, was examined byin situhybridization in the developing and adult murine olfactory system and compared with the expression of a known activator of HGF/SF, tissue-type plasminogen activator (tPA). In the developing olfactory canal, expression of bothc-metand tPA was observed in the olfactory neuroepithelium, whereas HGF/SF expression appeared to be confined to the mucosa adjacent to the neuroepithelium. During development of the olfactory bulb, HGF/SF and tPA were expressed within the rostral migratory pathway leading to the olfactory bulb, whereasc-metexpression was observed in the mitral cell layer (MCL) of the olfactory bulb and in the anterior olfactory nucleus. In the adult olfactory bulb, expression of HGF/SF was restricted to the periglomerular region of the glomerular layer, whereasc-metwas expressed in the MCL and olfactory nerve fiber layers (ONL). tPA expression in the adult olfactory bulb was observed in the ONL, MCL, and granule cell layers. Therefore, tPA expression was relatively coincident with the expression of HGF/SF and/orc-metin the appropriate projection patterns of the developing and adult olfactory system. In addition, antibodies against tPA inhibited the olfactory bulb extract-mediated cleavage of single-chain HGF/SF. These results suggest that tPA may play a regulatory role in the development and maintenance of the olfactory system by activating HGF/SF in the immediate vicinity of its receptor.
- University of Colorado Anschutz Medical Campus United States
- University of Colorado Cancer Center United States
DNA, Complementary, Hepatocyte Growth Factor, Age Factors, Gene Expression Regulation, Developmental, Receptor Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-met, Embryo, Mammalian, Olfactory Bulb, Olfactory Receptor Neurons, Mice, Inbred C57BL, Mice, Tissue Plasminogen Activator, Animals, Cloning, Molecular, In Situ Hybridization
DNA, Complementary, Hepatocyte Growth Factor, Age Factors, Gene Expression Regulation, Developmental, Receptor Protein-Tyrosine Kinases, Proto-Oncogene Proteins c-met, Embryo, Mammalian, Olfactory Bulb, Olfactory Receptor Neurons, Mice, Inbred C57BL, Mice, Tissue Plasminogen Activator, Animals, Cloning, Molecular, In Situ Hybridization
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