Genetic mapping of the Saccharomyces cerevisiae DNA polymerase I gene and characterization of a pol1 temperaturesensitive mutant altered in DNA primase-polymerase complex stability
Copyright policy )Genetic mapping of the Saccharomyces cerevisiae DNA polymerase I gene and characterization of a pol1 temperaturesensitive mutant altered in DNA primase-polymerase complex stability
The cloned DNA polymerase I gene has been used to map the POL1 locus on the left arm of chromosome XIV, between MET4 and TOP2. Temperature-sensitive mutants in POL1 have been obtained by in vitro mutagenesis of the cloned gene and in vivo replacement of the wild-type allele with the mutated copy. Physiological and biochemical characterization of one temperature-sensitive mutant (pol1-1) shows that cells shifted to the non-permissive temperature can complete one round of cell division and DNA replication before they arrest. Analysis of DNA polymerase I in crude extracts and in partially purified preparations indicates that the pol1-1 mutation results in a conformational change and affects the stability of the DNA primase-polymerase complex.
- University of Milan Italy
DNA Replication, Genes, Fungal, Mutation, Temperature, Chromosome Mapping, RNA Nucleotidyltransferases, DNA Primase, Saccharomyces cerevisiae, DNA Primase; DNA Polymerase I; Genes, Fungal; Temperature; RNA Nucleotidyltransferases; Chromosome Mapping; Mutation; DNA Replication; Saccharomyces cerevisiae, DNA Polymerase I
DNA Replication, Genes, Fungal, Mutation, Temperature, Chromosome Mapping, RNA Nucleotidyltransferases, DNA Primase, Saccharomyces cerevisiae, DNA Primase; DNA Polymerase I; Genes, Fungal; Temperature; RNA Nucleotidyltransferases; Chromosome Mapping; Mutation; DNA Replication; Saccharomyces cerevisiae, DNA Polymerase I
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