Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers
Copyright policy )Differential Effect of Calsequestrin Ablation on Structure and Function of Fast and Slow Skeletal Muscle Fibers
We compared structure and function of EDL and Soleus muscles in adult (4–6 m) mice lacking both Calsequestrin (CASQ) isoforms, the main SR Ca2+‐binding proteins. Lack of CASQ induced ultrastructural alterations in ~30% of Soleus fibers, but not in EDL. Twitch time parameters were prolonged in both muscles, although tension was not reduced. However, when stimulated for 2 sec at 100 hz, Soleus was able to sustain contraction, while in EDL active tension declined by 70–80%. The results presented in this paper unmask a differential effect of CASQ1&2 ablation in fast versus slow fibers. CASQ is essential in EDL to provide large amount of Ca2+ released from the SR during tetanic stimulation. In contrast, Soleus deals much better with lack of CASQ because slow fibers require lower Ca2+ amounts and slower cycling to function properly. Nevertheless, Soleus suffers more severe structural damage, possibly because SR Ca2+ leak is more pronounced.
- University of Padua Italy
- National Research Council Italy
- Stanford University United States
- University of Chieti-Pescara Italy
Male, Mice, Knockout, Analysis of Variance, Histocytochemistry, Body Weight, Calcium-Binding Proteins, Kaplan-Meier Estimate, Mice, Inbred C57BL, Mice, Muscle Fibers, Slow-Twitch, Muscle Tonus, Muscle Fibers, Fast-Twitch, Animals, Calsequestrin, Protein Isoforms, Female, Muscle Strength, Research Article, Muscle Contraction
Male, Mice, Knockout, Analysis of Variance, Histocytochemistry, Body Weight, Calcium-Binding Proteins, Kaplan-Meier Estimate, Mice, Inbred C57BL, Mice, Muscle Fibers, Slow-Twitch, Muscle Tonus, Muscle Fibers, Fast-Twitch, Animals, Calsequestrin, Protein Isoforms, Female, Muscle Strength, Research Article, Muscle Contraction
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