RNF8 Ubiquitylates Histones at DNA Double-Strand Breaks and Promotes Assembly of Repair Proteins
Copyright policy )RNF8 Ubiquitylates Histones at DNA Double-Strand Breaks and Promotes Assembly of Repair Proteins
Accumulation of repair proteins on damaged chromosomes is required to restore genomic integrity. However, the mechanisms of protein retention at the most destructive chromosomal lesions, the DNA double-strand breaks (DSBs), are poorly understood. We show that RNF8, a RING-finger ubiquitin ligase, rapidly assembles at DSBs via interaction of its FHA domain with the phosphorylated adaptor protein MDC1. This is accompanied by an increase in DSB-associated ubiquitylations and followed by accumulation of 53BP1 and BRCA1 repair proteins. Knockdown of RNF8 or disruption of its FHA or RING domains impaired DSB-associated ubiquitylation and inhibited retention of 53BP1 and BRCA1 at the DSB sites. In addition, we show that RNF8 can ubiquitylate histone H2A and H2AX, and that its depletion sensitizes cells to ionizing radiation. These data suggest that MDC1-mediated and RNF8-executed histone ubiquitylation protects genome integrity by licensing the DSB-flanking chromatin to concentrate repair factors near the DNA lesions.
- Danish Cancer Society Denmark
PROTEINS, Cell Survival, Cell Cycle Proteins, Models, Biological, Histones, Tumor Cells, Cultured, Humans, DNA Breaks, Double-Stranded, Phosphorylation, Adaptor Proteins, Signal Transducing, Binding Sites, Biochemistry, Genetics and Molecular Biology(all), BRCA1 Protein, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, DNA, Chromatin, Protein Structure, Tertiary, DNA-Binding Proteins, DNA Repair Enzymes, SIGNALING, Trans-Activators, Tumor Suppressor p53-Binding Protein 1, Protein Binding
PROTEINS, Cell Survival, Cell Cycle Proteins, Models, Biological, Histones, Tumor Cells, Cultured, Humans, DNA Breaks, Double-Stranded, Phosphorylation, Adaptor Proteins, Signal Transducing, Binding Sites, Biochemistry, Genetics and Molecular Biology(all), BRCA1 Protein, Intracellular Signaling Peptides and Proteins, Nuclear Proteins, DNA, Chromatin, Protein Structure, Tertiary, DNA-Binding Proteins, DNA Repair Enzymes, SIGNALING, Trans-Activators, Tumor Suppressor p53-Binding Protein 1, Protein Binding
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