AbstractIntrinsic Notch signaling in intestinal epithelial cells restricts secretory cell differentiation. In gut‐associated lymphoid tissue (GALT), stromal cells located beneath the follicle‐associated epithelium (FAE) abundantly express the Notch ligand delta‐like 1 (Dll1). Here, we show that mice lacking Rbpj—a gene encoding a transcription factor implicated in Notch signaling—in intestinal epithelial cells have defective GALT maturation. This defect can be attributed to the expansion of goblet cells, which leads to the down‐regulation of CCL20 in FAE. These data demonstrate that epithelial Notch signaling maintained by stromal cells contributes to the full maturation of GALT by restricting secretory cell differentiation in FAE.