The possible modulation of the glutamate transporter EAAC1 by a class A G protein‐coupled receptor was studied in transfected C6 glioma cells stably expressing the high‐affinity neurotensin receptor NTS1. Brief exposure (5 min) to neurotensin increased Na+‐dependent D‐[3H]aspartate uptake by about 70%. The effect of neurotensin was found to result from an increase in cell surface expression of EAAC1 and accordingly, cytochalasin D and colchicine were shown to block the effect of neurotensin on aspartate uptake, suggesting that the cytoskeleton participates in this regulation. Neither protein kinase C nor phosphatidylinositol 3‐kinase activities, two intracellular signaling pathways known to modulate EAAC1, was required for EAAC1‐mediated aspartate transport regulation by neurotensin. Together, these results provide evidence for an acute regulation of EAAC1 trafficking after activation of a G protein‐coupled receptor.