Near-atomic resolution visualization of human transcription promoter opening
Copyright policy )Near-atomic resolution visualization of human transcription promoter opening
In eukaryotic transcription initiation, a large multi-subunit pre-initiation complex (PIC) that assembles at the core promoter is required for the opening of the duplex DNA and identification of the start site for transcription by RNA polymerase II. Here we use cryo-electron microscropy (cryo-EM) to determine near-atomic resolution structures of the human PIC in a closed state (engaged with duplex DNA), an open state (engaged with a transcription bubble), and an initially transcribing complex (containing six base pairs of DNA-RNA hybrid). Our studies provide structures for previously uncharacterized components of the PIC, such as TFIIE and TFIIH, and segments of TFIIA, TFIIB and TFIIF. Comparison of the different structures reveals the sequential conformational changes that accompany the transition from each state to the next throughout the transcription initiation process. This analysis illustrates the key role of TFIIB in transcription bubble stabilization and provides strong structural support for a translocase activity of XPB.
- Georgia State University United States
- Lawrence Berkeley National Laboratory United States
- University of California, Berkeley United States
- Northwestern University United States
- University of California, San Francisco United States
Models, Molecular, DNA-Binding Proteins (mesh), Transcription Elongation, Genetic, Protein Conformation, General Science & Technology (science-metrix), TFII (mesh), Transcription Factors, TFII, Models, Hela Cells (mesh), Promoter Regions, Genetic, Transcription Initiation, Genetic, Cryoelectron Microscopy (mesh), Humans (mesh), 51 Physical Sciences (for-2020), Generic health relevance (hrcs-hc), Biological Sciences, DNA-Binding Proteins, Molecular (mesh), Multiprotein Complexes (mesh), Physical Sciences, DNA Helicases (mesh), RNA Polymerase II, Transcription Initiation, General Science & Technology, 1.1 Normal biological development and functioning, Movement, 610, Saccharomyces cerevisiae, HeLa Cells (mesh), Promoter Regions, Genetic, Genetics, Humans, Saccharomyces cerevisiae (mesh), 1.1 Normal biological development and functioning (hrcs-rac), TFII, 31 Biological Sciences (for-2020), Protein Conformation (mesh), Genetics (rcdc), Cryoelectron Microscopy, RNA Polymerase II (mesh), DNA Helicases, Molecular, DNA, Movement (mesh), 540, 3101 Biochemistry and Cell Biology (for-2020), Multiprotein Complexes, Genetic (mesh), Biochemistry and Cell Biology, Generic health relevance, DNA (mesh), Transcription Elongation, Transcription Factors, HeLa Cells
Models, Molecular, DNA-Binding Proteins (mesh), Transcription Elongation, Genetic, Protein Conformation, General Science & Technology (science-metrix), TFII (mesh), Transcription Factors, TFII, Models, Hela Cells (mesh), Promoter Regions, Genetic, Transcription Initiation, Genetic, Cryoelectron Microscopy (mesh), Humans (mesh), 51 Physical Sciences (for-2020), Generic health relevance (hrcs-hc), Biological Sciences, DNA-Binding Proteins, Molecular (mesh), Multiprotein Complexes (mesh), Physical Sciences, DNA Helicases (mesh), RNA Polymerase II, Transcription Initiation, General Science & Technology, 1.1 Normal biological development and functioning, Movement, 610, Saccharomyces cerevisiae, HeLa Cells (mesh), Promoter Regions, Genetic, Genetics, Humans, Saccharomyces cerevisiae (mesh), 1.1 Normal biological development and functioning (hrcs-rac), TFII, 31 Biological Sciences (for-2020), Protein Conformation (mesh), Genetics (rcdc), Cryoelectron Microscopy, RNA Polymerase II (mesh), DNA Helicases, Molecular, DNA, Movement (mesh), 540, 3101 Biochemistry and Cell Biology (for-2020), Multiprotein Complexes, Genetic (mesh), Biochemistry and Cell Biology, Generic health relevance, DNA (mesh), Transcription Elongation, Transcription Factors, HeLa Cells
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