Identification and characterisation of a nuclear localisation signal in the SMN associated protein, Gemin4
Copyright policy )Identification and characterisation of a nuclear localisation signal in the SMN associated protein, Gemin4
Gemin4 is a ubiquitously expressed multifunctional protein that is involved in U snRNP assembly, apoptosis, nuclear/cytoplasmic transportation, transcription, and RNAi pathways. Gemin4 is one of the core components of the Gemin-complex, which also contains survival motor neuron (SMN), the seven Gemin proteins (Gemin2-8), and Unrip. Mutations in the SMN1 gene cause the autosomal recessive disorder spinal muscular atrophy (SMA). Although the functions assigned to Gemin4 predominantly occur in the nucleus, the mechanisms that mediate the nuclear import of Gemin4 remain unclear. Here, using a novel panel of Gemin4 constructs we identify a canonical nuclear import sequence (NLS) in the N-terminus of Gemin4. The Gemin4 NLS is necessary and independently sufficient to mediate nuclear import of Gemin4. This is the first functional NLS identified within the SMN-Gemin complex.
- Peninsula College of Medicine and Dentistry United Kingdom
- University of Missouri Health System United States
- Plymouth University United Kingdom
- University of Exeter United Kingdom
- University of Missouri United States
Cell Nucleus, Cytoplasm, Nuclear Localization Signals, Active Transport, Cell Nucleus, RNA-Binding Proteins, Nerve Tissue Proteins, SMN Complex Proteins, Ribonucleoproteins, Small Nuclear, Survival of Motor Neuron 1 Protein, Minor Histocompatibility Antigens, Mutation, Humans, Cyclic AMP Response Element-Binding Protein, HeLa Cells
Cell Nucleus, Cytoplasm, Nuclear Localization Signals, Active Transport, Cell Nucleus, RNA-Binding Proteins, Nerve Tissue Proteins, SMN Complex Proteins, Ribonucleoproteins, Small Nuclear, Survival of Motor Neuron 1 Protein, Minor Histocompatibility Antigens, Mutation, Humans, Cyclic AMP Response Element-Binding Protein, HeLa Cells
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