The scaffolding protein CASK mediates the interaction between rabphilin3a and β‐neurexins
Copyright policy )The scaffolding protein CASK mediates the interaction between rabphilin3a and β‐neurexins
CASK, a member of the membrane‐associated guanylate kinase (MAGUK) superfamily, binds to the carboxyl‐terminus of β‐neurexins on the intracellular side of the presynaptic membrane. The guanylate kinase‐like (GUK) domains of MAGUKs lack kinase activities, but might be important for mediating specific protein–protein interaction. By a yeast two‐hybrid approach, we identified an interaction between the GUK domain of CASK and the C2B domain of rabphilin3a, a presynaptic protein involved in synaptic vesicle exocytosis. The interaction was confirmed by in vitro GST pull‐down and co‐immunoprecipitation assays. It was proposed that presynaptic vesicles might be guided to the vicinity of points of exocytosis defined by β‐neurexins via the interaction between rabphilin3a–CASK–β‐neurexins.
- Shanghai Institutes for Biological Sciences China (People's Republic of)
- Center for Excellence in Molecular Cell Science China (People's Republic of)
- Chinese Academy of Sciences China (People's Republic of)
Rabphilin3a, Recombinant Fusion Proteins, Blotting, Western, Vesicular Transport Proteins, Nerve Tissue Proteins, Rabphilin-3A, CASK, Exocytosis, Two-Hybrid System Techniques, Synaptic vesicle exocytosis, Animals, Protein Isoforms, Yeast two-hybrid system, Adaptor Proteins, Signal Transducing, Glutathione Transferase, β-Neurexin, Precipitin Tests, Protein Structure, Tertiary, rab GTP-Binding Proteins, COS Cells, Calcium-Calmodulin-Dependent Protein Kinases, Synaptic Vesicles, Nucleoside-Phosphate Kinase, Guanylate Kinases, Protein Binding
Rabphilin3a, Recombinant Fusion Proteins, Blotting, Western, Vesicular Transport Proteins, Nerve Tissue Proteins, Rabphilin-3A, CASK, Exocytosis, Two-Hybrid System Techniques, Synaptic vesicle exocytosis, Animals, Protein Isoforms, Yeast two-hybrid system, Adaptor Proteins, Signal Transducing, Glutathione Transferase, β-Neurexin, Precipitin Tests, Protein Structure, Tertiary, rab GTP-Binding Proteins, COS Cells, Calcium-Calmodulin-Dependent Protein Kinases, Synaptic Vesicles, Nucleoside-Phosphate Kinase, Guanylate Kinases, Protein Binding
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