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In vitro regeneration of Anacardium occidentale from shoot tip and basal part

Authors: Yah Gwladys Gnamien; Bi Tra Jean-Innocent Nanti; Tchoa Kone; Brahima André Soumahoro; Mongomaké Kone;

In vitro regeneration of Anacardium occidentale from shoot tip and basal part

Abstract

The culture of cashew (Anacardiumoccidentale L.) is the main source of income for populations in northern Cote d'Ivoire, with an estimated production of 725 000 tonnes in 2017, but the average yield remains low likely due to the lack of elite planting material and hence use of unselected plant material by mostfarmers. For mass propagation of such a material, in vitro methods are necessary. Unfortunately, it is difficult to obtain surviving explants from mature plants grown in the field, whereby explants from seedlings obtained by in vitro germination are the most suitable for micropropagation of cashew.The objective of this study was to propagate under in vitroconditionselite plants of Anacardiumoccidentaleto be used as planting material. In Nangui Abrogoua University laboratory,shoot tip and basal part explants derived from vitroplants of 16-day-old were transferred onto Murashige and Skoog (MS) medium containing different concentrations of cytokinins.After one month of culture, the induced shoots were placed onto different strengths of MS medium withvarious concentrations of sucrose and auxin. The highest number of buds (9) was recorded with the basal explants on medium supplemented withThidiazuron (TDZ) at 0.01 mg/l. The highest shoots (3 cm) were obtained with these sameexplantson a medium without growth regulators. A½ MS with 60 g/l of sucrose and 5 mg/l of IBA induced the highest rooting percentage (72%) and number of roots (4 roots) in a short time (16 days).

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
5
Top 10%
Average
Average
gold