Nob1p is required for biogenesis of the 26S proteasome and degraded upon its maturation in Saccharomyces cerevisiae
Copyright policy )Nob1p is required for biogenesis of the 26S proteasome and degraded upon its maturation in Saccharomyces cerevisiae
Nob1p is a nuclear protein that forms a complex with the 19S regulatory particle of the 26S proteasome and with uncharacterized nuclear protein Pno1p. Overexpression of NOB1 overrode the defects in maturation of the 20S proteasome of ump1Δ cells, and temperature-sensitive nob1 and pno1 mutants exhibited defects in the processing of the β subunits and in the assembly of the 20S and the 26S proteasomes. A defect in eitherNOB1 or PNO1 caused accumulation of newly formed Pre6p in the cytoplasm, whereas Pre6p of the ump1Δ strain accumulated in the nucleus irrespective of the temperature. Here we present a model proposing that (1) Nob1p serves as a chaperone to join the 20S proteasome with the 19S regulatory particle in the nucleus and facilitates the maturation of the 20S proteasome and degradation of Ump1p, and (2) Nob1p is then internalized into the 26S proteasome and degraded to complete 26S proteasome biogenesis.
- University of Tokyo Japan
Adenosine Triphosphatases, Proteasome Endopeptidase Complex, Molecular Sequence Data, Gene Expression Regulation, Developmental, Nuclear Proteins, Saccharomyces cerevisiae, Polymerase Chain Reaction, DNA-Binding Proteins, Fungal Proteins, Protein Transport, Microscopy, Fluorescence, Gene Expression Regulation, Fungal, Mutagenesis, Site-Directed, Amino Acid Sequence, Carrier Proteins, DNA, Fungal, DNA Primers, Molecular Chaperones, Peptide Hydrolases, Protein Binding
Adenosine Triphosphatases, Proteasome Endopeptidase Complex, Molecular Sequence Data, Gene Expression Regulation, Developmental, Nuclear Proteins, Saccharomyces cerevisiae, Polymerase Chain Reaction, DNA-Binding Proteins, Fungal Proteins, Protein Transport, Microscopy, Fluorescence, Gene Expression Regulation, Fungal, Mutagenesis, Site-Directed, Amino Acid Sequence, Carrier Proteins, DNA, Fungal, DNA Primers, Molecular Chaperones, Peptide Hydrolases, Protein Binding
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