Studies on the role of the metK gene product of Escherichia coli K‐12
Copyright policy )Studies on the role of the metK gene product of Escherichia coli K‐12
SummaryWe show here that the metK gene is essential to the growth of Escherichia coli K‐12 and can be deleted only in the presence of a rescue plasmid carrying a functional metK gene. When metK expression was limited, genomic DNA methylation decreased and cell division was hampered. Through primer extension, the transcription start site of metK was located at 140 bp upstream of the translation start site. The frequently used metK84 mutant has been shown to carry an A(r)G transition in the −10 region of the metK promoter. This accounts for its low level of S‐adenosylmethionine (SAM) synthetase and SAM deficiency.
- Concordia University Canada
- Concordia University Wisconsin United States
S-Adenosylmethionine, Base Sequence, Transcription, Genetic, Molecular Sequence Data, Methionine Adenosyltransferase, DNA Methylation, Culture Media, Glucose, Mutation, Escherichia coli, Promoter Regions, Genetic
S-Adenosylmethionine, Base Sequence, Transcription, Genetic, Molecular Sequence Data, Methionine Adenosyltransferase, DNA Methylation, Culture Media, Glucose, Mutation, Escherichia coli, Promoter Regions, Genetic
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).59 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!