PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1
Copyright policy )PP2A activates brassinosteroid-responsive gene expression and plant growth by dephosphorylating BZR1
When brassinosteroid levels are low, the GSK3-like kinase BIN2 phosphorylates and inactivates the BZR1 transcription factor to inhibit growth in plants. Brassinosteroid promotes growth by inducing dephosphorylation of BZR1, but the phosphatase that dephosphorylates BZR1 has remained unknown. Here, using tandem affinity purification, we identified protein phosphatase 2A (PP2A) as a BZR1-interacting protein. Genetic analyses demonstrated a positive role for PP2A in brassinosteroid signalling and BZR1 dephosphorylation. Members of the B' regulatory subunits of PP2A directly interact with BZR1's putative PEST domain containing the site of the bzr1-1D mutation. Interaction with and dephosphorylation by PP2A are enhanced by the bzr1-1D mutation, reduced by two intragenic bzr1-1D suppressor mutations, and abolished by deletion of the PEST domain. This study reveals a crucial function for PP2A in dephosphorylating and activating BZR1 and completes the set of core components of the brassinosteroid-signalling cascade from cell surface receptor kinase to gene regulation in the nucleus.
- University of California System United States
- Carnegie Institution of Washington United States
- University of Stavanger Norway
- CARNEGIE INSTITUTION OF WASHINGTON United States
- Carnegie Institution for Science United States
DNA-Binding Proteins (mesh), Arabidopsis, 11 Medical and Health Sciences (for), Medical and Health Sciences, Plant Growth Regulators, Gene Expression Regulation, Plant, Two-Hybrid System Techniques (mesh), Arabidopsis (mesh), Sequence Alignment (mesh), Protein Phosphatase 2, Phosphorylation, 3101 Biochemistry and cell biology (for-2020), Triazoles (mesh), Developmental Biology (science-metrix), Plant Growth Regulators (mesh), Nuclear Proteins, Biological Sciences, Plants, Genetically Modified (mesh), Plants, Genetically Modified, 06 Biological Sciences (for), DNA-Binding Proteins, Protein Phosphatase 2 (mesh), Signal Transduction, 570, Molecular Sequence Data, Genetically Modified, Arabidopsis Proteins (mesh), Article, Molecular Sequence Data (mesh), Nuclear Proteins (mesh), Two-Hybrid System Techniques, Genetics, Amino Acid Sequence, 580, 31 Biological Sciences (for-2020), Genetics (rcdc), Arabidopsis Proteins, Phosphorylation (mesh), Plant, Amino Acid Sequence (mesh), Triazoles, 3101 Biochemistry and Cell Biology (for-2020), Gene Expression Regulation, Signal Transduction (mesh), Biochemistry and Cell Biology, Plant (mesh), Sequence Alignment, Developmental Biology
DNA-Binding Proteins (mesh), Arabidopsis, 11 Medical and Health Sciences (for), Medical and Health Sciences, Plant Growth Regulators, Gene Expression Regulation, Plant, Two-Hybrid System Techniques (mesh), Arabidopsis (mesh), Sequence Alignment (mesh), Protein Phosphatase 2, Phosphorylation, 3101 Biochemistry and cell biology (for-2020), Triazoles (mesh), Developmental Biology (science-metrix), Plant Growth Regulators (mesh), Nuclear Proteins, Biological Sciences, Plants, Genetically Modified (mesh), Plants, Genetically Modified, 06 Biological Sciences (for), DNA-Binding Proteins, Protein Phosphatase 2 (mesh), Signal Transduction, 570, Molecular Sequence Data, Genetically Modified, Arabidopsis Proteins (mesh), Article, Molecular Sequence Data (mesh), Nuclear Proteins (mesh), Two-Hybrid System Techniques, Genetics, Amino Acid Sequence, 580, 31 Biological Sciences (for-2020), Genetics (rcdc), Arabidopsis Proteins, Phosphorylation (mesh), Plant, Amino Acid Sequence (mesh), Triazoles, 3101 Biochemistry and Cell Biology (for-2020), Gene Expression Regulation, Signal Transduction (mesh), Biochemistry and Cell Biology, Plant (mesh), Sequence Alignment, Developmental Biology
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