Immunohistochemically detected expression of Skp2, p27kip1, and p-p27 (Thr187) in patients with cholangiocarcinoma
Copyright policy )Immunohistochemically detected expression of Skp2, p27kip1, and p-p27 (Thr187) in patients with cholangiocarcinoma
The paper was aimed to detect the expression of Skp2, p27(kip1) (p27), and p-p27 (Thr187) in patients with cholangiocarcinoma (CCA). Western blot and immunohistochemistry were used to analyze the expression and subcellular localization of p27, Skp2, and p-p27 (Thr187) in tissue specimens of 53 patients with CCA and 10 with chronic proliferative cholangitis (CPC). Besides, the relationships of p27, Skp2, and p-p27 (Thr187) with clinicopathologic findings were examined, followed by analysis of the relationships of p27 expression with p-p27 (Thr187) and Skp2 in CCA tissue. The expression of p27 was lower in CCA than CPC tissue, and the expression of Skp2 and p-p27 (Thr187) was higher in CCA than CPC tissue (P < 0.05). The positive p27 and Skp2 proteins were mainly expressed in nucleus and cytoplasm of CCA, and p-p27 (Thr187) was only observed in nucleus. The expression of p27, Skp2, and p-p27 (Thr187) was closely associated with tumor grade and TNM stage (P < 0.05). A significantly negative correlation between the expression of p27 and Skp2 (r = -0.480, P < 0.05) and between the expression of p27 and p-p27 (Thr187) (r = -0.387, P < 0.05) was observed. Skp2-dependent proteasomal degradation of p27 plays role in the malignant transformation of CCA. Besides, the expression of p27, Skp2, and p-p27 (Thr187) may serve as markers for the progression of CCA.
- Huazhong University of Science and Technology China (People's Republic of)
- Tongji Hospital China (People's Republic of)
Adult, Male, Middle Aged, Cholangiocarcinoma, Gene Expression Regulation, Neoplastic, Biomarkers, Tumor, Humans, Female, Phosphorylation, S-Phase Kinase-Associated Proteins, Cyclin-Dependent Kinase Inhibitor p27, Aged, Cell Proliferation, Neoplasm Staging
Adult, Male, Middle Aged, Cholangiocarcinoma, Gene Expression Regulation, Neoplastic, Biomarkers, Tumor, Humans, Female, Phosphorylation, S-Phase Kinase-Associated Proteins, Cyclin-Dependent Kinase Inhibitor p27, Aged, Cell Proliferation, Neoplasm Staging
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