Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51
Copyright policy )Valine 1532 of human BRC repeat 4 plays an important role in the interaction between BRCA2 and RAD51
The breast cancer susceptibility protein BRCA2 is essential for recombinational DNA repair. BRCA2 specifically binds to RAD51 via eight BRC repeat motifs and delivers RAD51 to double-stranded DNA breaks. In this study, a mammalian two-hybrid assay and competitive ELISA showed that the interaction between BRC repeat 4 (BRC4) and RAD51 was strengthened by the substitution of a single BRC4 amino acid from valine to isoleucine (V1532I). However, the cancer-associated V1532F mutant exhibited very weak interaction with RAD51. This study used a comparative analysis of BRC4 between animal species to identify V1532 as an important residue that interacts with RAD51.
- Hokkaido Bunkyo University Japan
- Advanced Science Research Center Japan
- Kitasato University Japan
- Iwate University Japan
- Hokkaido University Japan
BRCA2 Protein, BRC repeat, Valine, BRCA2, Canine, Neoplasm Proteins, Amino Acid Substitution, Two-Hybrid System Techniques, 649, RAD51, Animals, Humans, Mutant Proteins, Rad51 Recombinase, Homologous recombination, Protein Binding, Repetitive Sequences, Nucleic Acid
BRCA2 Protein, BRC repeat, Valine, BRCA2, Canine, Neoplasm Proteins, Amino Acid Substitution, Two-Hybrid System Techniques, 649, RAD51, Animals, Humans, Mutant Proteins, Rad51 Recombinase, Homologous recombination, Protein Binding, Repetitive Sequences, Nucleic Acid
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