Molecular cloning and expression of aminopeptidase A isoforms from rat hippocampus
Copyright policy )Molecular cloning and expression of aminopeptidase A isoforms from rat hippocampus
The full-length cDNA encoding aminopeptidase A (APAL) was cloned from a rat hippocampus cDNA library. A short variant aminopeptidase A (APAS), produced by deletion, was also cloned. In the case of APAL, the longest open reading frame encodes 945 amino acid residues with a calculated molecular mass of 108 kDa, and the deduced amino acid sequence shows 76, 86 and 78% identity with its human, murine and porcine counterparts, respectively. Rat aminopeptidase A mRNAs were detected in the kidney, liver, heart and brain by Northern blot analysis. When overexpressed in COS-1 cells, APAL shows apparent aminopeptidase A activity, whereas APAS does not.
Brain Chemistry, DNA, Complementary, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Gene Expression, Glutamyl Aminopeptidase, Transfection, Aminopeptidases, Hippocampus, Rats, Isoenzymes, COS Cells, Animals, Amino Acid Sequence, Cloning, Molecular, Sequence Alignment, Gene Library
Brain Chemistry, DNA, Complementary, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Gene Expression, Glutamyl Aminopeptidase, Transfection, Aminopeptidases, Hippocampus, Rats, Isoenzymes, COS Cells, Animals, Amino Acid Sequence, Cloning, Molecular, Sequence Alignment, Gene Library
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