A single PXXP motif in the C‐terminal region of srGAP3 mediates binding to multiple SH3 domains
Copyright policy )A single PXXP motif in the C‐terminal region of srGAP3 mediates binding to multiple SH3 domains
The Slit‐Robo GTPase‐activating protein 3 (srGAP3) has been implicated in different critical aspects of neuronal development. These findings have mainly been based on the characterisation of the three conserved globular N‐terminal domains, while the function of the C‐terminal region (CTR) is still unknown. We show that this predicted unstructured region acts as an adaptor by binding to the endocytic proteins Amphiphysin, Endophilin‐A2, Endophilin‐A1, as well as the Ras signalling protein Grb2. All these interactions depend on a single proline‐rich motif in the CTR and the Src‐homology 3 domains of the binding partners. Via these interactions srGAP3 could link receptor signalling events to the endocytic machinery.
- Max Planck Institute for Developmental Biology Germany
- Max Planck Institute for Biology Germany
- Max Planck Society Germany
WAVE-associated RacGAP protein, Amino Acid Motifs, GTPase-Activating Proteins, Nerve Tissue Proteins, Endocytosis, Protein–protein interaction, src Homology Domains, HEK293 Cells, Isothermal titration calorimetry, Humans, Mental disorder GAP, SH3 domain (adaptor), Adaptor Proteins, Signal Transducing, GRB2 Adaptor Protein, Protein Binding, Signal Transduction
WAVE-associated RacGAP protein, Amino Acid Motifs, GTPase-Activating Proteins, Nerve Tissue Proteins, Endocytosis, Protein–protein interaction, src Homology Domains, HEK293 Cells, Isothermal titration calorimetry, Humans, Mental disorder GAP, SH3 domain (adaptor), Adaptor Proteins, Signal Transducing, GRB2 Adaptor Protein, Protein Binding, Signal Transduction
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