Cloning of 5′-flanking region and a polymorphic CTT trinucleotide repeat within 5′-untranslated region of mouse R-type calcium channel α1-subunit (Cchra1) gene, and its genetic mapping
Copyright policy )Cloning of 5′-flanking region and a polymorphic CTT trinucleotide repeat within 5′-untranslated region of mouse R-type calcium channel α1-subunit (Cchra1) gene, and its genetic mapping
The 5'-flanking region of the mouse R-type calcium channel (Cchra1) gene was cloned, and a transcriptional start point (tsp) was determined by rapid amplification of 5'-cDNA end (5'RACE) method. The putative promoter region of the gene contained no obvious TATA or CCAAT element in the expected positions, but multiple putative binding sites for transcriptional factors, such as Sp1, AP-1, AP-2, AP-3, EGR-1, EGR-2, NF-kappaB and HIP1, were detected. We found the existence of a tandem CTT trinucleotide repeat within the 5'-untranslated region (UTR) of the gene, and its polymorphism between C57BL/6J and Mus spretus. Using this polymorphism, the Cchra1 was mapped to the region of chromosome 1 where the synteny to human chromosome 1q was conserved.
- Eisai Japan
Male, Binding Sites, Polymorphism, Genetic, Base Sequence, Molecular Sequence Data, Chromosome Mapping, Polymerase Chain Reaction, Mice, Inbred C57BL, Muridae, Mice, Species Specificity, Animals, Humans, Female, Amino Acid Sequence, Calcium Channels, Cloning, Molecular, Promoter Regions, Genetic, DNA Primers, Transcription Factors
Male, Binding Sites, Polymorphism, Genetic, Base Sequence, Molecular Sequence Data, Chromosome Mapping, Polymerase Chain Reaction, Mice, Inbred C57BL, Muridae, Mice, Species Specificity, Animals, Humans, Female, Amino Acid Sequence, Calcium Channels, Cloning, Molecular, Promoter Regions, Genetic, DNA Primers, Transcription Factors
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