Differential suppressive effect of promyelocytic leukemia protein on the replication of different subtypes/strains of influenza A virus
Copyright policy )Differential suppressive effect of promyelocytic leukemia protein on the replication of different subtypes/strains of influenza A virus
Promyelocytic leukemia protein (PML) plays an important role in the defense against a number of viruses, including influenza A virus. However, the sensitivity of influenza A virus subtypes/strains to PML is unknown. We investigated the role of PML in the replication of different influenza A virus subtypes/strains using pan-PML knock-down A549 cells and PML-VI-overexpressed MDCK cells. We found that (i) depletion of pan-PML by siRNA rendered A549 cells more susceptible to influenza A virus strains PR8(H1N1) and ST364(H3N2), but not to strains ST1233(H1N1), Qa199(H9N2) and Ph2246(H9N2); (ii) overexpression of PML-VI in MDCK cells conferred potent resistance to PR8(H1N1) infection, while lacked inhibitory activity to ST1233(H1N1), ST364(H3N2), Qa199(H9N2) and Ph2246(H9N2). Our results suggest that the antiviral effect of PML on influenza A viruses is viral subtype/strain specific.
- Shantou University China (People's Republic of)
- Key Laboratory of Guangdong Province China (People's Republic of)
- Chinese Academy of Medical Sciences & Peking Union Medical College China (People's Republic of)
- Shantou University Medical College China (People's Republic of)
- PEKING UNION MEDICAL COLLEGE China (People's Republic of)
Influenza A Virus, H3N2 Subtype, Tumor Suppressor Proteins, Nuclear Proteins, Promyelocytic Leukemia Protein, Virus Replication, Dogs, Influenza A Virus, H1N1 Subtype, Cell Line, Tumor, Gene Knockdown Techniques, Influenza A Virus, H9N2 Subtype, Animals, Humans, Transcription Factors
Influenza A Virus, H3N2 Subtype, Tumor Suppressor Proteins, Nuclear Proteins, Promyelocytic Leukemia Protein, Virus Replication, Dogs, Influenza A Virus, H1N1 Subtype, Cell Line, Tumor, Gene Knockdown Techniques, Influenza A Virus, H9N2 Subtype, Animals, Humans, Transcription Factors
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