Purification and molecular weight determination of glucose-6-phosphate dehydrogenase and malic enzyme from mouse and Drosophila
Copyright policy )Purification and molecular weight determination of glucose-6-phosphate dehydrogenase and malic enzyme from mouse and Drosophila
Abstract A facile two-step procedure was employed for simultaneous purification of glucose-6-phosphate dehydrogenase and malic enzyme from mouse (strain DBA 2J ) and Drosophila melanogaster. This involved the use of an 8-(6-aminohexyl)-amino-2′,5′-ADP-Sepharsoe affinity column chromatography followed by DEAE-Sephadex chromatography. The native and subunit molecular weights of these two homogeneous enzymes were determined by gel-filtration chromatography and SDS-polyacrylamide gel electrophoresis. From this study, it was concluded that the two enzymes are tetrameric and have native molecular weights between 200,000 and 280,000 in both species.
- National Institutes of Health United States
- Research Triangle Park Foundation United States
- Jackson Laboratory United States
- National Institute of Health Pakistan
- National Institute of Environmental Health Sciences United States
570, Macromolecular Substances, Glucosephosphate Dehydrogenase, Kidney, Biochemistry, Bioassays, Chromatography, Affinity, Organs:, Adenosine Diphosphate, Molecular Weight, Mice, Drosophila melanogaster, Strains:, Liver, Malate Dehydrogenase, Metabolism:, Animals, Techniques:
570, Macromolecular Substances, Glucosephosphate Dehydrogenase, Kidney, Biochemistry, Bioassays, Chromatography, Affinity, Organs:, Adenosine Diphosphate, Molecular Weight, Mice, Drosophila melanogaster, Strains:, Liver, Malate Dehydrogenase, Metabolism:, Animals, Techniques:
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