Heterologous SH3-p85β inhibits influenza A virus replication
Copyright policy )Heterologous SH3-p85β inhibits influenza A virus replication
Abstract Phosphatidylinositol 3-kinase (PI3K)/Akt signalling pathway can support the replication of influenza A virus through binding of viral NS1 protein to the Src homology 3 (SH3) domain of p85β regulatory subunit of PI3K. Here we investigated the effect of heterologously overexpressed SH3 on the replication of different influenza A virus subtypes/strains, and on the phosphorylation of Akt in the virus-infected cells. We found that heterologous SH3 reduced replication of influenza A viruses at varying degrees in a subtype/strain-dependent manner and SH3 overexpression reduced the induction of the phosphorylation of Akt in the cells infected with PR8(H1N1) and ST364(H3N2), but not with ST1233(H1N1), Ph2246(H9N2), and Qa199(H9N2). Our results suggest that interference with the NS1-p85β interaction by heterologous SH3 can be served as a useful antiviral strategy against influenza A virus infection.
- Key Laboratory of Guangdong Province China (People's Republic of)
- Shantou University Medical College China (People's Republic of)
- Shantou University China (People's Republic of)
Research, Down-Regulation, Infectious and parasitic diseases, RC109-216, Viral Nonstructural Proteins, Virus Replication, Cell Line, src Homology Domains, Phosphatidylinositol 3-Kinases, Protein Subunits, Infectious Diseases, Dogs, Influenza A virus, Virology, Influenza, Human, Animals, Humans, Phosphorylation, Proto-Oncogene Proteins c-akt, Protein Binding
Research, Down-Regulation, Infectious and parasitic diseases, RC109-216, Viral Nonstructural Proteins, Virus Replication, Cell Line, src Homology Domains, Phosphatidylinositol 3-Kinases, Protein Subunits, Infectious Diseases, Dogs, Influenza A virus, Virology, Influenza, Human, Animals, Humans, Phosphorylation, Proto-Oncogene Proteins c-akt, Protein Binding
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