Distinct roles of profilin in cell morphological changes: microspikes, membrane ruffles, stress fibers, and cytokinesis
Copyright policy )Distinct roles of profilin in cell morphological changes: microspikes, membrane ruffles, stress fibers, and cytokinesis
Here we report the functional importance of profilin in various actin‐mediated morphological changes using H119E mutant profilin I, which is deficient only in actin binding. In the case of actin‐protrusive structures from the plasma membrane, H119E‐profilin was shown to suppress the formation of Cdc42‐induced actin microspikes and Rac‐induced membrane ruffles. Conversely, Rho‐induced stress fiber formation seemed to occur independently of H119E‐profilin introduction. Furthermore, H119E‐profilin blocked cleavage furrow ingression and subsequent adhesion to the substratum during cell division, a process in which actin plays indispensable roles.
- University of Tokyo Japan
Profilin, Cell Cycle Proteins, Mice, Profilins, Contractile Proteins, GTP-Binding Proteins, Animals, Humans, cdc42 GTP-Binding Protein, Actin, Cytokinesis, Platelet-Derived Growth Factor, Cell Cycle, Cell Membrane, Microfilament Proteins, 3T3 Cells, Actins, Recombinant Proteins, rac GTP-Binding Proteins, Small G protein, Cell morphology, Mutation, Lysophospholipids, Cell Division, HeLa Cells
Profilin, Cell Cycle Proteins, Mice, Profilins, Contractile Proteins, GTP-Binding Proteins, Animals, Humans, cdc42 GTP-Binding Protein, Actin, Cytokinesis, Platelet-Derived Growth Factor, Cell Cycle, Cell Membrane, Microfilament Proteins, 3T3 Cells, Actins, Recombinant Proteins, rac GTP-Binding Proteins, Small G protein, Cell morphology, Mutation, Lysophospholipids, Cell Division, HeLa Cells
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