Molecular Architecture of the 40S⋅eIF1⋅eIF3 Translation Initiation Complex
Copyright policy )Molecular Architecture of the 40S⋅eIF1⋅eIF3 Translation Initiation Complex
Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. We present X-ray structures of all major components of the minimal, six-subunit Saccharomyces cerevisiae eIF3 core. These structures, together with electron microscopy reconstructions, cross-linking coupled to mass spectrometry, and integrative structure modeling, allowed us to position and orient all eIF3 components on the 40S⋅eIF1 complex, revealing an extended, modular arrangement of eIF3 subunits. Yeast eIF3 engages 40S in a clamp-like manner, fully encircling 40S to position key initiation factors on opposite ends of the mRNA channel, providing a platform for the recruitment, assembly, and regulation of the translation initiation machinery. The structures of eIF3 components reported here also have implications for understanding the architecture of the mammalian 43S preinitiation complex and the complex of eIF3, 40S, and the hepatitis C internal ribosomal entry site RNA.
ISSN:0092-8674
ISSN:1097-4172
Cell, 158 (5)
- ETH Zurich Switzerland
- University of California, San Francisco United States
- University of Konstanz Germany
- ETH-Zurich Switzerland
- Department of Biology Switzerland
Models, Molecular, Saccharomyces cerevisiae Proteins, Eukaryotic Initiation Factor-3, Molecular Sequence Data, Eukaryotic Initiation Factor-1, Hepacivirus, Saccharomyces cerevisiae, Crystallography, X-Ray, Article, Animals, Humans, Amino Acid Sequence, Peptide Chain Initiation, Translational, info:eu-repo/classification/ddc/570, Mammals, Ribosome Subunits, Small, Eukaryotic, Biochemistry, Genetics and Molecular Biology(all), Microscopy, Electron, Ribonucleoproteins, Dimerization, Sequence Alignment
Models, Molecular, Saccharomyces cerevisiae Proteins, Eukaryotic Initiation Factor-3, Molecular Sequence Data, Eukaryotic Initiation Factor-1, Hepacivirus, Saccharomyces cerevisiae, Crystallography, X-Ray, Article, Animals, Humans, Amino Acid Sequence, Peptide Chain Initiation, Translational, info:eu-repo/classification/ddc/570, Mammals, Ribosome Subunits, Small, Eukaryotic, Biochemistry, Genetics and Molecular Biology(all), Microscopy, Electron, Ribonucleoproteins, Dimerization, Sequence Alignment
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