Wee1B, Myt1, and Cdc25 function in distinct compartments of the mouse oocyte to control meiotic resumption
Copyright policy )Wee1B, Myt1, and Cdc25 function in distinct compartments of the mouse oocyte to control meiotic resumption
After a long period of quiescence at dictyate prophase I, termed the germinal vesicle (GV) stage, mammalian oocytes reenter meiosis by activating the Cdc2–cyclin B complex (maturation-promoting factor [MPF]). The activity of MPF is regulated by Wee1/Myt1 kinases and Cdc25 phosphatases. In this study, we demonstrate that the sequestration of components that regulate MPF activity in distinct subcellular compartments is essential for their function during meiosis. Down-regulation of either Wee1B or Myt1 causes partial meiotic resumption, and oocytes reenter the cell cycle only when both proteins are down-regulated. Shortly before GV breakdown (GVBD), Cdc25B is translocated from the cytoplasm to the nucleus, whereas Wee1B is exported from the nucleus to the cytoplasm. These movements are regulated by PKA inactivation and MPF activation, respectively. Mislocalized Wee1B or Myt1 is not able to maintain meiotic arrest. Thus, cooperation of Wee1B, Myt1, and Cdc25 is required to maintain meiotic arrest and relocation of these components before GVBD is necessary for meiotic reentry.
- University of California, San Francisco United States
- University of California System United States
- UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
- Reproductive Science Center United States
- Inje University Korea (Republic of)
Biomedical and clinical sciences, 1.1 Normal biological development and functioning, Cells, Maturation-Promoting Factor, Active Transport, Cell Nucleus, Down-Regulation, Cell Cycle Proteins, Medical and Health Sciences, Mice, Underpinning research, Animals, cdc25 Phosphatases, Research Articles, Cells, Cultured, Cell Nucleus, Cultured, Biomedical and Clinical Sciences, Nuclear Proteins, Biological Sciences, Protein-Tyrosine Kinases, Cyclic AMP-Dependent Protein Kinases, Active Transport, Cell Compartmentation, DNA-Binding Proteins, Biological sciences, Meiosis, Protein Transport, Reproductive Medicine, Mesothelin, Oocytes, Biochemistry and Cell Biology, Developmental Biology, Transcription Factors
Biomedical and clinical sciences, 1.1 Normal biological development and functioning, Cells, Maturation-Promoting Factor, Active Transport, Cell Nucleus, Down-Regulation, Cell Cycle Proteins, Medical and Health Sciences, Mice, Underpinning research, Animals, cdc25 Phosphatases, Research Articles, Cells, Cultured, Cell Nucleus, Cultured, Biomedical and Clinical Sciences, Nuclear Proteins, Biological Sciences, Protein-Tyrosine Kinases, Cyclic AMP-Dependent Protein Kinases, Active Transport, Cell Compartmentation, DNA-Binding Proteins, Biological sciences, Meiosis, Protein Transport, Reproductive Medicine, Mesothelin, Oocytes, Biochemistry and Cell Biology, Developmental Biology, Transcription Factors
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