Cloning and sequencing of the 5′ region of the human glucose‐6‐phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells
Copyright policy )Cloning and sequencing of the 5′ region of the human glucose‐6‐phosphatase gene: transcriptional regulation by cAMP, insulin and glucocorticoids in H4IIE hepatoma cells
We have cloned and sequenced the first 1.2 kb of the 5′ region of the human glucose‐6‐phosphatase gene. Transfection of H4IIE hepatoma cells with the 1.2 kb fragment fused to a luciferase reporter gene demonstrated both basal and hormone responsive luciferase activity. Dexamethasone increased and insulin decreased luciferase activity. Insulin and dibutyryl cyclic AMP both significantly decreased activity in the presence of dexamethasone.
- University of Dundee United Kingdom
Base Sequence, Transcription, Genetic, Molecular Sequence Data, Transfection, Dexamethasone, Gene Expression Regulation, Enzymologic, Gene regulation, Rats, Liver Neoplasms, Experimental, Bucladesine, Genes, Reporter, cAMP, Glucose-6-Phosphatase, Tumor Cells, Cultured, Animals, Humans, Insulin, Cloning, Molecular, Promoter Regions, Genetic, Glucose-6-phosphatase
Base Sequence, Transcription, Genetic, Molecular Sequence Data, Transfection, Dexamethasone, Gene Expression Regulation, Enzymologic, Gene regulation, Rats, Liver Neoplasms, Experimental, Bucladesine, Genes, Reporter, cAMP, Glucose-6-Phosphatase, Tumor Cells, Cultured, Animals, Humans, Insulin, Cloning, Molecular, Promoter Regions, Genetic, Glucose-6-phosphatase
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