Cloning and Characterization of Dfak56, a Homolog of Focal Adhesion Kinase, in Drosophila melanogaster
Copyright policy )Cloning and Characterization of Dfak56, a Homolog of Focal Adhesion Kinase, in Drosophila melanogaster
The focal adhesion kinase (FAK) protein-tyrosine kinase plays important roles in cell adhesion in vertebrates. Using polymerase chain reaction-based cloning strategy, we cloned a Drosophila gene that is homologous to the vertebrate FAK family of protein-tyrosine kinases. We designated this gene Dfak56 and characterized its gene product. The overall protein structure and deduced amino acid sequence of Dfak56 show significant similarity to those of FAK and PYK2. Dfak56 has in vitro autophosphorylation activity at tyrosine residues. Expression of the Dfak56 mRNA and the protein was observed in the central nervous system and the muscle-epidermis attachment site in the embryo, where Drosophila position-specific integrins are localized. The results suggest that like FAK in vertebrates, Dfak56 functions downstream of integrins. Dfak56 was tyrosine-phosphorylated upon integrin-dependent attachment of the cell to the extracellular matrix. We conclude that the Dfak56 tyrosine kinase is involved in integrin-mediated cell adhesion signaling and thus is a functional homolog of vertebrate FAK.
- Osaka University Japan
- University of Tsukuba Japan
- Tokyo Ariake University of Medical and Health Sciences Japan
- Osaka Gakuin University Japan
- University of Tokyo Japan
Integrins, Molecular Sequence Data, Fluorescent Antibody Technique, Gene Expression Regulation, Developmental, Protein-Tyrosine Kinases, Phosphoproteins, Gene Expression Regulation, Enzymologic, Extracellular Matrix, Drosophila melanogaster, Focal Adhesion Kinase 1, Focal Adhesion Protein-Tyrosine Kinases, Cell Adhesion, Animals, Drosophila Proteins, Amino Acid Sequence, Cloning, Molecular, Phosphorylation, Phosphotyrosine, Cell Adhesion Molecules, In Situ Hybridization
Integrins, Molecular Sequence Data, Fluorescent Antibody Technique, Gene Expression Regulation, Developmental, Protein-Tyrosine Kinases, Phosphoproteins, Gene Expression Regulation, Enzymologic, Extracellular Matrix, Drosophila melanogaster, Focal Adhesion Kinase 1, Focal Adhesion Protein-Tyrosine Kinases, Cell Adhesion, Animals, Drosophila Proteins, Amino Acid Sequence, Cloning, Molecular, Phosphorylation, Phosphotyrosine, Cell Adhesion Molecules, In Situ Hybridization
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